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:

 
 





:: # water # microbiology
    
# water # microbiology
10  2020    00:15 
   
water microbiology
========================
:
=============================
Escherichia 200 :
: :
:
===========
:
1- 25 ( )
2- 1 9 10/1
3- 10/1 1 2 10/ 1 1 10/1 9 100/1
4- 100/1 1 2 100/1 1 100/1 9 100/1
5- 1 100/1 2 100/1
6-
7- 37 48
8- " 30-300 " 1 ɡ
9- : 
============
1 =
1- ( 30 300 )
: " " , .. ...
E.coli. .
E.coli :
=====================================
1. Presumptive:
2. Contirmed Test:
ɡ 3-2 . E.coli E.coli E.coli 
EosineMethylene blue E.coli .
3. completed Test:
====================
))
( E.coli +

2)

3) E.M.B
Eosine Methylene Blue E.coli
Completed Test: 
====================
E.coli 37 48 +. .
http://elte.prompt.hu/sites/default/files/tananyagok/PracticalMicrobiology/book.pdf

https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/316838/mdwpart1.pdf

http://www.env.gov.nl.ca/env/waterres/training/adww/qualitydist/distribution_sys2_micro_march.pdf

http://users.rowan.edu/~wagnerf/efgb/h2omicrolab.pdf

http://microbiollogy.blogspot.com/

* : 
========================
1- :
==========
:
- Psychrophililes : 15-10 .
- Psychrotrophic : .
- () Mesophiles : 25-37 32 .
- Thermophiles : 45 .
2- :
=====
.
3- Moisture :
============
Water Activity (aw) .
4- pH :
===================
7 .
5- :
=============
- Aerobes : . 
- Anaerobes : .
- Facultative Anaerobs : .
- Microaerophiles : .
http://archive.bio.ed.ac.uk/jdeacon/microbes/shape.htm

 
===============
CULTUR MEDIA
*******************
, . , .
..
1- Chemically defined media :
=====================================
" " , , , .
2- Chemically nodefined method :
===========================================
, , , , .
:
======================
1- "" Solid media :
=====================
.
2- Solid-reversible to liquid :
================================
, .
3- Semisolid media :
========================
.
4- Liquid media :
==================
, . :
1- Enriched media :
=====================
, ,
Serum agar Milk agar Blood agar
2- Selective media :
=======================
, , ,
3- Deferential media :
======================
, , , .
4- Biological assay media
5- Media for eneumeration of bacteria
6- Media for characterization of bacteria
:
===================
:
 
====
, .
Beef extract
================
, , , , , , .
Yeast extract
==================
, , .
Water-
========
, , .
Solidifying agents :
======================
.
..
 
======
, , , .
Agar agar
==============
, , 42- 45, 98, .
Silica-
=======
,
* *
ǡ ɡ ϡ . ǡ ɡ () . . .
:
=============
1. ( )
2.  
3. .
4. .
5. .
6. .
7.  
8. ( )
9. .
10. : .
11.  
12. : .
13. .
14. .
15. .
16. .
17. 10 100 .
:
,
:
1.
2.
3. Ribowclease
4. .
* :
================
:
: Streak-plate method .
1- 70%.
2- 50 .
3- . 
4- (1) .
5- .
: Pour plate method.
1- 2 50.
2- (1) .
3- (1) (2) .
4- (1) (1) , (2) (2).
5- .
6- (1) (2) .
Mucoid Nonmucoid , .
===================
. .
:
:
1.
2.
3.
4.  
5.
: .
: .
6.
7.
() ........ Xanthomonas .
() Pseudomonas .
:
1.
2.
3.
Ý-
ȝ-
ʝ- 
:
========================
1.
Ý-
ȝ-
ʝ-
˝- ( )
̝-
͝- 
2.
3.
....................
: __
:
1-
- - - - - -
2_
- - - - -
3_
- - - - -
4_
- -
5_
- -
6_
- - - -
7_ ...............
:
1-
- - -
2_
- - - - - -
3_
- -
:
=========================
1_
- - -
- ( )
-
2_
- - -
3_
- - - - -
Bacterial Growth curve (cycle)
1. Lag phase 
.
2. ( ) Logarithmic Growth Phase
.
3. Stationary phase
.
4. Death (Decline) Phase
.
1- .
2- .
3- .
4- .

:
==========
ɡ  
===============================
http://archive.bio.ed.ac.uk/jdeacon/microbes/shape.htm
========================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/myxococc.htm
==============================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/xanthan.htm#Top
============================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/bt.htm#Top
=======================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/airborne.htm
============================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/winograd.htm
=============================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/cyano.htm#Top
===========================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/index.htm
============================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/proteus.htm#Top
============================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/control.htm#Top
============================================
http://archive.bio.ed.ac.uk/jdeacon/microbes/penicill.htm
http://archive.bio.ed.ac.uk/jdeacon/microbes/thermo.htm#Top
http://archive.bio.ed.ac.uk/jdeacon/microbes/nitrogen.htm

   
========================
http://www.building-tech-mag.com/researches/14/derasat-an-jawdat-meyah.pdf
 
============
=============
. ( 10 ) 100 . (10 ) . . 50 1:10 1:10000. ߡ . ( ). . .
=============================
ATP. . ATP Luciferase Luminometer. . ATP .
======
. . ɡ 30 -300 . 30 300 . . (1:10 ,1:100 ,1:1000 etc) . Plate count agar MacConkey agar . 22 C 24 37 C 24 . . . ɡ .
=======
. (). .
======
. . . cfu/ml . .
=======================
MacConkey agar 
=======================
. ( ) crystal violet ( ) , neutral red ( ) .
ENDO ,Dipotassium phosphate ѡ basic fuchsin Salmonella typhi . , coliform . .
mFC 
=======
. Rosolic acid coliforms ɡ non-enteric Aniline blue coliforms PH .
TYEA 
========
Tryptone Yeast extract L-arabinose. (22c and 36 C) .
:
===========================================
1- Total plate count at 37C/ 24h
2- Total plate count at 22C/ 48h
3- Total Coliform
4- E.coli
5- Faecal streptococci
==================================
http://pdf.usaid.gov/pdf_docs/Pnady300.pdf

 
======
https://tools.thermofisher.com/content/sfs/brochures/BR-70541-Water-Quality-Analysis-BR70541-EN.pdf
 
    
     
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